JOURNAL HOME CME HOME THIS MONTH PAST ISSUES ETOC COLLECTIONS AUTHORS REVIEWERS EDITORIAL BOARD FEEDBACK RSS HELP
		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a colleague Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Buljubasic, N. Articles by Bosnjak, Z. J. Search for Related Content PubMed PubMed Citation Articles by Buljubasic, N. Articles by Bosnjak, Z. J.

Halothane Reduces Release of Adenosine, Inosine, and Lactate with Ischemia and Reperfusion in Isolated Hearts

Anesthesiology Research Laboratory, Departments of Anesthesiology, Physiology, and Pharmacology, Medical College of Wisconsin and Veterans Administration Medical Center, Milwaukee, Wisconsin

Abstract

We investigated the protective effects of halothane on cardiac function of isolated hearts during global hypoperfusion and reperfusion by examining halothane's effects on altering coronary flow, myocardial oxygen utilization (MVO₂), and release of adenosine (ADE), inosine (INO), and lactate (LAC). Isolated perfused guinea pig hearts were divided into three groups of perfusion at 25% (14 mm Hg), 10% (5.5 mm Hg), and 0% (no perfusion) from control perfusion pressure (PP, 55 mm Hg). Each of these PP groups was subdivided into three subgroups and perfused without halothane (control), with 0.23 ± 0.01 mM (0.74%) halothane, or with 0.51 ± 0.01 mM (1.65%) halothane. Halothane was present 10 min before reducing PP, during reduced PP (30 min), and for 10 min after reducing PP. Hypoperfusion was followed by 40 min of reperfusion at the control (100%, 55 mm Hg) PP. An additional group of control hearts was followed for the same period without reducing PP or perfusing with halothane. Exposure to 0.74% and 1.65% halothane, before reducing PP, decreased MVO₂ and percent oxygen extraction (% O₂E), but produced no significant change in coronary flow or release of ADE, ISO, or LAC. During early hypoperfusion (10 min) at 25% PP, 1.65% halothane significantly reduced release of ADE, INO, and LAC. During late hypoperfusion (40 min) the differences in LAC release diminished, but release of ADE and INO remained lower in the 1.65% halothane group. With early reperfusion there was a large increase in release of these metabolites, that was dependent on the decrease in perfusion pressure. The release of INO during reperfusion was reduced by halothane; however, ADE release increased with halothane suggesting less conversion of ADE to INO. The release of LAC was not affected by halothane. The reduction of cardiac work effected by halothane either before, during, or after graded reductions in PP may decrease the loss of purine substrate so that synthesis of high energy phosphates is less impaired. This study suggests that our earlier finding that halothane improved contractile function and reduced the severity of dysrhythmias in isolated hearts following graded reductions in PP is due, at least in part, to a decrease in oxygen demand relative to oxygen supply, resulting in a decrease in purine release.

This article has been cited by other articles:

				J. A. Symons and P. S. Myles Myocardial protection with volatile anaesthetic agents during coronary artery bypass surgery: a meta-analysis Br. J. Anaesth., August 1, 2006; 97(2): 127 - 136. [Abstract] [Full Text] [PDF]

				M. Tonkovic-Capin, G. J. Gross, Z. J. Bosnjak, J. S. Tweddell, C. M. Fitzpatrick, and J. E. Baker Delayed cardioprotection by isoflurane: role of KATP channels Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H61 - H68. [Abstract] [Full Text] [PDF]

				J. An, S. G. Varadarajan, E. Novalija, and D. F. Stowe Ischemic and anesthetic preconditioning reduces cytosolic [Ca2+] and improves Ca2+ responses in intact hearts Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1508 - H1523. [Abstract] [Full Text] [PDF]

				B. Siegmund, W. Schlack, Y. V. Ladilov, C. Balser, and H. M. Piper Halothane Protects Cardiomyocytes Against Reoxygenation-Induced Hypercontracture Circulation, December 16, 1997; 96(12): 4372 - 4379. [Abstract] [Full Text]

				H. Habazettl, B. W. Palmisano, B. M. Graf, D. L. Roerig, Z. J. Bosnjak, and D. F. Stowe IMPROVEMENT IN FUNCTIONAL RECOVERY OF THE ISOLATED GUINEA PIG HEART AFTER HYPERKALEMIC REPERFUSION WITH ADENOSINE J. Thorac. Cardiovasc. Surg., January 1, 1996; 111(1): 74 - 84. [Abstract] [Full Text]

Anesthesia & Analgesia® is published for the International Anesthesia Research Society® by Lippincott Williams & Wilkins with the assistance of Stanford University Libraries' HighWire Press®. Copyright 2006 by the International Anesthesia Research Society. Online ISSN: 1526-7598   Print ISSN: 0003-2999